Microscope slide for enhanced analysis of DNA damage using the comet assay.
نویسندگان
چکیده
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منابع مشابه
Detection of DNA damage and identification of UV-induced photoproducts using the CometAssay kit.
We introduce the first commercially available comet assay for the detection and quantification of DNA damage in individual eukaryotic cells. The major difficulty of the comet assay is the preparation of the slides needed to immobilize the samples throughout the lysis and electrophoretic procedures. The CometAssay kit uses a proprietary technology to precoat glass microscope slides to allow dire...
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Background: ˝Radioadaptive Response˝ is well-documented phenomenon appeared in low dose ionizing radiation received in vitro and in vivo. Occupational exposure has always been a great concern for radiation workers therefore this study was performed to study radioadaptive response in terms of residual DNA double strand breaks as an endpoint in peripheral blood leukocytes of occupationally expose...
متن کاملAutomatic analysis of slides processed in the Comet assay.
In recent years the Comet assay (or single cell gel electrophoresis assay) has been established as a rapid and sensitive method for the detection of DNA damage. For early genotoxicity screening of new chemical entities in industrial toxicology, the Comet assay is more and more used for assessment of the DNA damaging potential of a test compound. In order to increase compound screening throughpu...
متن کاملNovel method for the high-throughput processing of slides for the comet assay
Single cell gel electrophoresis (the comet assay), continues to gain popularity as a means of assessing DNA damage. However, the assay's low sample throughput and laborious sample workup procedure are limiting factors to its application. "Scoring", or individually determining DNA damage levels in 50 cells per treatment, is time-consuming, but with the advent of high-throughput scoring, the limi...
متن کاملDNA Damage in Leukocytes from Fanconi Anemia (FA) Patients and Heterozygotes Induced by Mitomycin C and Ionizing Radiation as Assessed by the Comet and Comet-FISH Assay
Background: Lymphocytes of Fanconi anemia (FA) show an increased sensitivity to the alkylating agents such as mitomycin C (MMC), but their responses to gamma-irradiation is controversial. The extent of DNA damage in leukocytes of FA patients following irradiation and MMC treatment was studied at cellular and single chromosome level. Methods: DNA damage induced by gamma-rays and MMC was measure...
متن کاملCytogenetic damage induced by crude oil in Anodonta cygnea (mollusca,bivalvia) assessed by the comet assay and micronucleus test
Crude oil is enriched in polycyclic aromatic hydrocarbons (PAHs). Many PAH analogs have proved to potentially damage DNA. DNA damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. In this research the comet assay and micronucleus (MN) test were used to detect DNA damages and cytogenetic changes following crude oil expos...
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عنوان ژورنال:
- BioTechniques
دوره 25 1 شماره
صفحات -
تاریخ انتشار 1998